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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Nat Neurosci. 2011 Apr 24;14(6):727–735. doi: 10.1038/nn.2804

Figure 2.

Figure 2

The increase in γMEAN from GluA1/GluA2/stargazin transfected cells in is not caused by a stargazin-induced increase in a subpopulation of homomeric GluA1 receptors. A. Normalized current-voltage relationship for GluA1±stargazin and GluA1/GluA2±stargazin (mean ± SEM). B. Rectification ratios (RR) were calculated as the ratio of current amplitude at +40 mV, which reflects primarily GluA1/GluA2 heteromeric receptor current, to that at −60 mV (mean ± SEM; n = 8–18 cells for each condition). From the stargazin-induced change in the rectification ratio, we estimate that co-transfection with GluA1, GluA2, and stargazin leads to 96% GluA1/GluA2 receptors and 4% homomeric GluA1 (Methods). C. The predicted weighted mean conductance is plotted as a function of an unknown GluA1/GluA2 conductance according to equations 4–6 (see Methods). The broken lines show how the weighted γMEAN changes with progressively increasing FA1 using the conductance of GluA1-S831A and GluA1-S831A/GluA2. The thick black line is the weighted γMEAN when FA1 is the 4% of homomeric GluA1-S831A receptors with γMEAN of 9.7 pS determined at +40 mV, and 96% GluA1-S831A/GluA2. The thick gray line shows weighted γMEAN for GluA1-S831E with γ of 14.1pS determined at +40 mV and 96% GluA1-S831E/GluA2. D. The relationship between the change in weighted γMEAN induced by the GluA1-S831E alone in a mixed population of GluA1 (4%) and GluA1/GluA2 (96%) receptors over a range of GluA1/GluA2 conductance values. The dotted line shows that the experimentally observed γMEAN is more than that predicted from a conductance increase in only the GluA1 subpopulation.