Table 2.

Sensitivity of multiplex microassay and fitting parameters

Toxin	Goodness of 4P fita, R2	Curve steepness (mL/ng)	IC50 (ng/mL)b	LOD (ng/mL)
DON (buffer)	0.9994	−1.181	35	9
DON (maize)	0.9966	−1.138	113	21
DON (wheat)	0.9922	−1.048	62	17
ZEN (buffer)	0.9997	−2.154	10	4
ZEN (maize)	0.9900	−1.410	29	16
ZEN (wheat)	0.9979	−2.392	19	10

^aGoodness of the four-parameter model fit to the calibration curve

^bIC₅₀ value derived from the four-parameter model fit of the calibration curve