Table 3.
The number of m/z values discriminated in the active androgenic fractions (agonistic or antagonistic and corresponding reversed phase [RP] and normal phase [NP] fractions) corresponding to identifications steps 1–10, separated into: the number of base-peak-framed m/z values from SIEVE; the number of m/z values with a ratio between 10–100 and >100 between control and sample; tentatively identified compound (some m/z values have multiple possibilities); available compounds for the confirmation steps; the analytically confirmed and the bioassay-confirmed compounds that represent the key toxicants in the sample
| Bioassay activity | Fraction | SIEVE m/z values | Ratio >100 m/z values | Tentatively identified | Available compounds | Analytical confirmed | Bioassay-confirmed |
|---|---|---|---|---|---|---|---|
| Identification step | 2 | 3–4 | 7 | 8 | 8 | 9 | |
| Agonistic | RP2NP7 | 1,002 | 38 | 25 | 8 | 2 | 1 |
| RP3NP7 | 2,915 | 62 | 7 | 1 | 0 | 0 | |
| Antagonistic | RP2NP6 | 1,206 | 35 | 6 | 2 | 1 | 1 |
| RP3NP2 | 3,309 | 26 | 2 | 0 | 0 | 0 | |
| RP3NP5 | 1,141 | 42 | 42 | 7 | 5 | 4 | |
| RP3NP6 | 2,940 | 26 | 4 | 2 | 1 | 1 | |
| RP4NP2 | 291 | 1 | 1 | 0 | 0 | 0 | |
| RP4NP7 | 2,003 | 29 | 8 | 2 | 1 | 1 | |
| Total | 14,807 | 259 | 95 | 22 | 10 | 8 | |