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. Author manuscript; available in PMC: 2011 May 27.
Published in final edited form as: Adv Protein Chem Struct Biol. 2009 Nov 27;78:99–146. doi: 10.1016/S1876-1623(08)78004-0

Figure 4. Yeast two-hybrid assay for binding to PCNA and hRev1-CTD.

Figure 4

Each segment of hPol η, hPol ι, hPol κ or hRev1 was inserted into pLexA (BD) vector and the entire region of PCNA or a region (951–1251) of hRev1 was inserted into pB42AD vector. Amino acid sequences of the inserted segments are shown in the right, in which conserved residues are underlined and altered sequences are shown in Italic. “No” indicates empty vector. Experiments were performed as described previously (Ohashi et al., 2009).

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