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. Author manuscript; available in PMC: 2011 Nov 26.
Published in final edited form as: Nature. 2011 May 26;473(7348):484–488. doi: 10.1038/nature10016

Figure 3.

Figure 3

Applications of SiMPull assay. (a–c) β2AR-YFP pull-down. (d–f) MAVS pull-down. Mitochondrial fraction from cells over-expressing YFP-MAVS was added either directly or after detergent solubilization (g–i) mTORC1 pull-down. Lysate from cells expressing Flag-mTOR, HA-Raptor or both was applied on chambers with Flag antibody, and probed through primary antibody against HA and labeled secondary antibody. (j–m) Endogenous PKA-AKAP complex pull-down from mouse brain extract. (k) WB shows AKAP immunoprecipitation with PKA antibody. (l) Immunofluorescence images of AKAP150 pulled down through PKA antibody. (c, f, i, m) show Nf. Scale bars are 5 µm. Error bars denote s. d. (n > 20).