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. Author manuscript; available in PMC: 2012 Mar 30.
Published in final edited form as: J Am Chem Soc. 2011 Mar 4;133(12):4359–4366. doi: 10.1021/ja107775w

Figure 3.

Figure 3

UV-Vis spectra of DsbB(C41S) from pH 5.53 to 7.85. DsbB in 25 mM Tris, pH 7.8 was titrated with 1 M MES (pH 5.5) to adjust the pH. The spectral change was complete within 30 s. Each spectrum was corrected for the change in volume upon addition of MES. Inset shows the absorbance at maximum 488 nm as a function of pH. pKa is determined to be 6.62 ± 0.02 by numerical fitting of the titration curve.