Skip to main content
. Author manuscript; available in PMC: 2011 May 27.
Published in final edited form as: J Immunol. 2010 May 7;184(12):7207–7218. doi: 10.4049/jimmunol.0903487

Figure 7. EP4 receptor regulates inflammatory gene expression in microglia isolated from adult mouse brain.

Figure 7

Microglia were isolated by density gradient centrifugation from 2-3 mo C57B7 male mice administered saline or LPS +/- EP4 agonist (AE1-329 0.3mg/kg) or vehicle (A), and from 2-3 mo Cd11bCre:EP4+/+ and Cd11bCre:EP4f/f mice 6 hours and 24 hours after LPS (B). (A) Significant increases in microglial expression of COX-2, iNOS, IL-6, TNF-α, and gp91phox were observed in wild type mice in response to LPS, but these increases were significantly blunted with co-treatment with EP4 agonist (#p<0.01; (*p<0.05; **p<0.01; n=6-8 mice per group). (B) Proinflammatory gene expression is elevated in Cd11bCre:EP4+/+ and Cd11bCre:EP4f/f microglia at 6 hours after LPS; however, increased gene expression persists at 24h in microglia isolated from Cd11bCre:EP4f/f mice as compared to Cd11bCre:EP4+/+ control mice. Gene expression does not return to basal levels for COX-2, IL-1ß, and TNF-α at 24h in Cd11bCre:EP4f/f microglia, and is significantly increased beyond the 6 hour level for iNOS at this late time point (*p<0.05; **p<0.01; n=4-8 per group).