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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Biorheology. 2011 Jan 1;48(1):1–35. doi: 10.3233/BIR-2011-0579

Fig. 9.

Fig. 9

Long tethers facilitate neutrophil rolling at high shear stress [Adapted from Sundd, P. et al. Nat Methods 7, 821-824 (2010) with permission]. (a) qDF micrograph of a DiI-labeled neutrophil rolling on P-selectin substrate processed to saturation to reveal anchorage points of long tethers behind the footprint. (b) 3D reconstruction of the footprint shown in (a) reveals z position of tether anchorage points above the P-selectin substrate which is equal to the length of the stressed P-selectin-PSGL-1 bonds holding the tether anchorage point to the substrate. P-selectin 20 molecules/μm2. Wall shear stress 0.8 Pa. Flow is from left to right. Scale bar 10 μm. Arrows mark tether anchorage points.