TABLE 1.
Partial list of transcription factors whose phosphorylation increased in Sirt1-KO MEF cells
The phospho-antibody microarray identified a list of transcription factors whose phosphorylation states increased in MEFs derived from Sirt1-KO mice. The signal intensities of phosphorylated and total forms of each protein were determined, and the ratio (phospho/total) of each protein was calculated between Sirt1-KO and WT MEFs. 95% confidence interval (CI) was determined to demonstrate the significance of the signal alteration for each transcription factor. The impact of site-specific phosphorylation on the biological function of these transcription factors is shown. This information can be found on line.
| Phosphorylation sites | Ratio (Sirt1-KO/WT) | 95% CI | Function |
|---|---|---|---|
| NF-κB-p65 (Ser(P)-529) | 6.54 | 5.39–7.68 | Activation |
| Elk1 (Thr(P)-417) | 5.25 | 3.50–7.00 | Activation |
| NF-κB-p65 (Ser(P)-468) | 4.03 | 3.04–5.03 | Slight inhibition |
| NF-κB-p65 (Ser(P)-536) | 3.68 | 2.15–5.20 | Activation |
| Smad2/3 (Thr(P)-8) | 3.67 | 3.09–4.24 | Activation |
| GATA1 (Ser(P)-310) | 3.62 | 3.35–3.88 | Activation |
| NF-κB-p105/p50 (Ser(P)-932) | 3.28 | 1.27–5.30 | Activation |
| FOXO1A/3A (Ser(P)-322/325) | 3.28 | 2.79–3.78 | Inactivation |
| CREB (Ser(P)-129) | 2.64 | 2.26–3.03 | Activation |