FIGURE 4.

Effect of tyrphostin AG1024 on BRET between IR and PTP1B-D181A-Y46F. A, HEK-293 cells were transfected with IR-Rluc and YFP-PTP1B-D181A-Y46F. 48 h after transfection, cells were preincubated for 1 h in the absence (−) or presence (+) of 100 μm AG1024. BRET measurements were performed in real time, after addition of either 100 nm insulin (Ins) or vehicle. Results are representative of five independent experiments. B, graphic representation of BRET signals measured 20 min after addition of 100 nm insulin. Results are the mean ± S.E. of five independent experiments. ***, p < 0.001 when compared with cells not treated with AG1024.