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. 2011 Apr 13;286(22):19381–19391. doi: 10.1074/jbc.M111.218040

FIGURE 5.

FIGURE 5.

STK17A knockdown results in decreased sensitivity to cisplatin in NT2/D1 cells. A, real time PCR analysis of STK17A expression in control NT2/D1 cells and cells transduced with two independent STK17A shRNA lentiviruses. NT2 represents mock-transduced cells, and PLK represents cells transduced with empty pLKO.1 vector. *, p < 0.01 compared with identically treated NT2/D1 control cells. B, Western analysis of STK17A expression in NT2/D1 cells stably transduced with pLKO.1 and STK17A shRNA lentiviruses. The band for STK17A is directly below the two nonspecific bands. C, dose response after 3-day cisplatin treatment of mock-transduced NT2/D1 cells or cells transfected with control or STK17A shRNA lentiviruses. Cell proliferation and survival were measured by the Cell-Titer Glo assay. *, p < 0.05; **, p < 0.01 compared with identically treated NT2/D1 control cells. Data points are the average of biological triplicates. Error bars are S.D. Data are representative of three independent experiments.