Constants for reactivation of OP-hAChE conjugates by lead reactivator RS41A and reference reactivators MINA, DAM, and 2-PAM
The maximal reactivation rate constant (k2), the Michaelis-type dissociation constant of OP-hAChE-oxime complex (Kox), and overall second order reactivation rate constant (kr) were calculated by nonlinear regression from plots presented in Fig. 3. Experiments were done in duplicate or triplicate at 37 °C in 0.10 m phosphate buffer, pH 7.4, and are summarized as shown in Fig. 3.