FIGURE 5.

IL-1β-stimulated up-regulation of HAS2-AS1 and HAS2 is mediated by Sp1 and Sp3, and TGF-β1-stimulated up-regulation of HAS2-AS1 and HAS2 is mediated by Smad2 and Smad3. Shown is the relative expression of HAS2 mRNA (A) and HAS2-AS1 RNA (B) in response to IL-1β (1 ng/ml) stimulation for 3 h and of HAS2 mRNA (C) and HAS2-AS1 RNA (D) following the addition of TGF-β1 (10 ng/ml) for 48 h. White bars, unstimulated PTCs, and black bars, cytokine-treated cells. Target gene siRNAs were used at 30 nm, and scrambled siRNA (SCR) was used at 60 nm. Data are shown from one of two replicate experiments, each carried out in triplicate, and error bars are S.D. *, p < 0.05.