FIGURE 6.

siRNA knockdown of HAS2-AS1 down-regulates HAS2 transcription. Shown is the relative expression of HAS2-AS1 RNA (A) and HAS2 mRNA (B) in response to the presence of IL-1β (1 ng/ml) for 3 h following transfection with three different siRNAs designed to knockdown HAS2-AS1 RNA. White bars, unstimulated PTCs; black bars, cytokine-treated cells. HAS2-AS1 siRNAs were used at 30 nm, and scrambled siRNA (SCR) was used at 30 nm. Data are shown from one of four replicate experiments, each carried out in triplicate, and error bars are S.D. *, p < 0.05.