FIGURE 8.

RNA duplex modeling and detection. A, computed minimum free energy secondary structure of the heterodimer of L-HAS2-AS1 RNA (green) and HAS2 mRNA (red) showing the duplex formed by regions of perfect complementarity. B, detection of ribonuclease- and deoxyribonuclease-resistant cytoplasmic RNA duplex by RT-PCR with primers designed to amplify transcription outside (a, 5′-cttcgagcagcccattgaac-3′ and 5′-agcctgtggaagactcagca-3′) or within (b, 5′-aaacagttgccctttgcatc-3′ and 5′-tgatttgtctctgcccatga-3′) the predicted region of L-HAS2-AS1/HAS2 heteroduplex formation.