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. 2011 Apr 7;286(22):19589–19596. doi: 10.1074/jbc.M110.197111

FIGURE 7.

FIGURE 7.

Pertussis toxin does not affect Gli-dependent transcription in MEFs. A, expression of gli1, ptc1, and the housekeeping gene S15 was analyzed by semiquantitative RT-PCR. Ptc1+/− MEFs were grown until fully confluent, pretreated with 100 ng/ml PTX, 0.5 μm KAAD-cyclopamine, or 15 μm LY294002 and then stimulated with 2.5 μg/ml Shh, 5 μm purmorphamine, or vehicle for 24 h in DMEM with 0.5% FBS. B, effect of PTX treatment in Ptc1+/− cells was also quantified by real-time PCR. C, effect of PTX, KAAD-cyclopamine, and LY294002 on the basal expression level of gli1 in Ptc1−/− and SMO−/− cells. D, expression of Gαz in membranes isolated from Ptc1+/− MEFs or platelets (positive control) by Western blot.