TABLE 2.

Role of PlGF-1 core residues involved in the interaction with AF, as elicited by structural analyses and docking model

LP, Limited Proteolysis; UVcl, UV cross-linking; Dock: docking. For the nature of interactions, PlGF-1/VEGFR-1 and the data reported in the last column (the numbers in parentheses represent the number of contacts with the VEGFR-1 residues indicated), see Ref. 31. For the decrease affinity by mutation in Ala, see Ref. 25.

PlGF residues	Type of analysis	Residues at 6 Å from AF (type of interaction)	Nature of interactions PlGF/VEGFR-1	Affinity decrease by mutation in Ala	VEGFR-1D2 putative intermolecular contacts
Pro-25	Dock			25%
Phe-26	Dock	aromatic π-π	Van der Waals		Pro-143(2), Leu-221(2)*
Gln-27	LP + Dock		polar	50%	Glu-141(3), Ile-142, Pro-143(9), Leu-204, Asn-219(3)
Gln-88	Dock	H-π	Van der Waals		Ile-142
Arg-97	UVcl + Dock
Pro-98	UVcl + Dock	H-bond (C7-OH group)		50%
Ser-99	UVcl + Dock	H-π
Tyr-100	UVcl + Dock	aromatic π-π	Van der Waals + polar	25%	Ile-142(10), Pro-143(2)