FIGURE 8.

MLIP is an alternatively spiced gene. A, RT-PCR was performed on mRNA isolated from mouse hearts using primers targeted to the 5′- and 3′-UTR of MLIP. The RT-PCR product was TA cloned into pCR-II plasmid (Invitrogen) and transformed into bacteria. Direct PCR was performed with primers targeting flanking regions of the MLIP insertion site amplified with each PCR product sequenced. Cloned MLIP cDNA length and predicted protein mass are identified for each of the bands (left and center columns). Calculated protein mass based on migration rates for each of the MLIP bands is presented in Fig. 6C (right column). B, based on these data, an alternative splice map of MLIP was constructed. The numbers below each splice map reflect the transcript size and predicted translated protein size.