TABLE 2.

Pre-steady state kinetic parameters for nucleotide insertion opposite a template guanine in wild-type BF and BF V713P mutant

See supplemental Fig. 4 online for experimental data and curve fits.

BF	Nascent base pair	KD	Kpol	Kpol/KD	Relative efficiencya
		μm	s−1	μm−1s−1
Wild type	dG:dCTP	21	52	2.3
	dG:dTTP	830	0.55	0.00066	2.6 × 10−4
V713P	dG:dCTP	12	2.8	0.24
	dG:dTTP	220	0.0036	0.000017	7 × 10−5

^a Ratio of k_pol/K_D values for dTTP vs dCTP incorporation opposite dG.