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. 2011 Apr 4;286(22):19789–19803. doi: 10.1074/jbc.M111.230094

FIGURE 2.

FIGURE 2.

Bacterial tubulin polymers formed under enhanced microtubule assembly conditions. A, electron micrograph of negatively stained BtubA/B polymers assembled in Pipes buffer with 200 g/liter Ficoll 70 and 0.1 mm GMPCPP at 37 °C. B, BtubA/B polymers assembled in Pipes buffer with 1 m sodium glutamate and 0.1 mm GMPCPP. C, microtubules assembled from calf brain αβ-tubulin in Pipes buffer with 200 g/liter Ficoll 70 and 0.1 mm GMPCPP. The bar corresponds to 100 nm for A–C. D, enlarged view (top panel) and computed diffractogram (bottom panel) of one BtubA/B polymer assembled in Ficoll. E, BtubA/B polymer in sodium glutamate and its diffractogram. F, enlarged αβ-tubulin microtubule assembled in Ficoll and its diffractogram. The spots indicated by the arrows corresponding to a characteristic 42-Å axial spacing between tubulin monomers along protofilaments and a 53-Å lateral spacing.

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