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Cooperative assembly of BtubA/B measured by sedimentation. A, polymer formation at varying concentrations of BtubA/B with 1 mm GTP in Tris-KCl at 25 °C, analyzed by SDS-PAGE (top panel) and quantified (bottom panel); the filled and open circles are pelleted and supernatant concentrations, respectively. The cross-hair is a control without Mg2+, the triangle is without GTP, and the inverted triangle is with 1 mm GDP. B, a similar experiment made with 0.1 mm GMPCPP.
