FIGURE 8.

Expression, assembly, and GTPase of bacterial tubulin chimera with eukaryotic tubulin loops. A, color-ranked recovery of purified BtubA/B chimera following expression in E. coli. B, light scattering assembly time courses of the different chimera in Tris-KCl buffer, 0.1 mm GTP, at 25 °C. C, GTPase activity of the chimeras plotted versus the time of half-depolymerization. The line is a least squares reciprocal fit to the data. D, electron micrographs of negatively stained polymers BtubA/B1MSβ chimera (10 μm) in Pipes microtubule assembly buffer with 0.1 mm GMPCPP at 30 °C. The bar indicates 200 nm.