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The FBDs of Fbxo2/NFB42 and Fbxo4 contain NESs. A, immunoblots of lysates from cells expressing Fbxo2 and Fbxo4 fusion proteins to Venus with anti-GFP antibodies. Images of the localization of the Venus fusions (green) of the WT and ΔF-box deletion mutants of Fbxo2 (B) and Fbxo4 (C) transfected into U2OS cells and visualized in live cells by fluorescence microscopy. Nuclei are stained by Hoechst 33342 (blue). The size bar in these images is 50 μm.
