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. 2011 Mar 4;286(22):19804–19815. doi: 10.1074/jbc.M111.220079

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Binding of Fbxo7 to Skp1 and CRM1 is mutually exclusive. A, immunoblots for in vitro synthesized T7-Fbxo7 (1–522), T7-Fbxo7 (169–398), and Skp1 (1–146) used as inputs for in vitro binding assays. Immunoblots of T7 and Skp1 for proteins present on GST and GST-CRM1 columns. In vitro synthesized Skp1 was titrated into a binding reaction of immobilized GST-CRM1 and in vitro synthesized T7-Fbxo7 (169–398) (B) or T7-Fbxo7 (1–522) (C) proteins. (D) Images of immunoblots for the indicated proteins on input and anti-Flag immunoprecipitated proteins from nuclear (Nuc) or cytoplasmic (Cyto) extracts of U2OS cells expressing Flag-Fbxo7 (1–522).

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