FIGURE 11.

Treatment with H₂O₂ increases Thr⁵⁰⁵ phosphorylation of PKCδ without affecting PKCδ expression. MN9D cells were incubated with 0.5 mm H₂O₂ for various time spans (3–24 h) and then were harvested for measurement of native PKCδ (A) or PKCδ Thr505 phosphorylation (B) levels by Western blot analysis. β-Actin was used as a loading control. Representative immunoblots are shown. con, control.