FIGURE 6.

Role of integrins during in vitro decidualization. A, MESC were subjected to in vitro decidualization for 24, 48, and 72 h, and the levels of integrin β1 and integrin β3 mRNAs were determined using real time PCR. B, MESC undergoing decidualization was analyzed by immunofluorescence using antibodies recognizing integrin β1. DAPI-stained nuclei are shown in blue. Bar, 100 μm. C, relative expression of mRNAs encoding integrins α1, α2, α6, and α7 in decidualizing MESC. D, MESC were transfected with 40 nm siCTRL or siRNA against integrin β1 siRNA (siITGB1). Relative expressions of integrin β1, PRP, ALP, and PR mRNAs are shown. Fold changes indicate gene expression levels in siITGB1-treated MESC relative to siCTRL-treated MESC after 72 h. All real time PCR measurements were performed in three independent trials, and statistically significant differences (p < 0.01) are indicated by *.