Skip to main content
. 2011 Apr 18;286(22):19880–19891. doi: 10.1074/jbc.M111.233080

FIGURE 5.

FIGURE 5.

Effect of SII and AngII on β-arrestin 2-bound Akt and GSK3β phosphorylation. A, schematic depicting the previously reported model for steady-state suppression of Akt activity in dopamine D2 receptor-β-arrestin 2 signalsomes (18) compared with the proposed model for Akt activation resulting from transient inhibition of signalsome PP2A activity (see text for explanation). B, representative immunoblots (IB) showing SII-induced phosphorylation of Akt-Thr-308 and its downstream target GSK3β-Ser-9 measured in FLAG-β-arrestin 2 immunoprecipitates (IP) from HEK-AT1AR cells isolated after stimulation with SII or AngII for the indicated times. For comparison, the lack of detectable ligand effect on the total cellular pools of phospho-Akt-Thr-308 and phospho-GSK3β-Ser-9 is shown in the lower panel. C, bar graphs depicting phospho-Akt-Thr-308 and phospho-GSK3β-Ser-9 normalized to the amount of FLAG-β-arrestin 2 in the immunoprecipitate. *, t test p < 0.05, greater than NS (n = 8 for Akt, n = 5 for GSK3β).