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. 2011 Apr 8;286(22):19982–19992. doi: 10.1074/jbc.M111.240077

FIGURE 5.

FIGURE 5.

SND1 suppresses apoptosis and regulates expression of ALDH3A1 and KiSS1. A, modified Boyden chamber invasion assays using control or SND1-KD LM2 and SCP28 cells. B, in vitro growth curves of control or SND1-KD LM2 and SCP28 cells. C–E, control or SND1-KD LM2 and SCP28 cells were treated with either Adriamycin (Adr) or ethanol (control) and stained for apoptosis induction with M30 CytoDEATH. Flow cytometry (C) and immunofluorescence analyses (E) were used to monitor the extent of apoptosis induction. P2 indicates the apoptosis-positive populations. D, quantified data from apoptosis assays based on flow cytometry. F, expression of ALDH3A1 and KiSS1 in control or SND1-KD LM2 cells was determined via quantitative RT-PCR. G and H, KiSS1 promoter activity was assessed via a luciferase reporter assay of control versus SND1-KD LM2 cells (G) or control versus HA-SND1-transfected 293T cells (H). Scale bar in E, 50 μm. *, p < 0.05; **, p < 0.01. Ctrl, control. Data represent average ± S.E.