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. 2011 Apr 20;286(22):20043–20053. doi: 10.1074/jbc.M111.218727

FIGURE 2.

FIGURE 2.

hnRNPs L and K have the majority of the silencing activity among the ESS1-associated proteins. A, the percentage of skipping of wild-type exon 4 in JSL1 cells upon individual knockdown of hnRNPs L, K, E, D, and PTB by transfection of the indicated amount of morpholino oligonucleotide. The average of multiple independent experiments is graphed. Corresponding S.D. and p values are shown in supplemental Table 1. Double asterisks indicate p value of less than 0.01. B, Western blots demonstrating knockdown from representative experiment used for graph in A. MO, morpholino oligonucleotide. C, the percentage of skipping of exon 5 in JSL1 cells. D, the percentage of skipping of exon 4 derivative lacking ESS1 done as for panel A. E, the percentage of skipping of exon 4 done as for panel A with 0.5 nmol of morpholino against each of the specified proteins alone or in combination. F, quantification of splicing of the endogenous CD45 gene in cells treated as in panel E. Statistics are shown in supplemental Table 1. The corresponding Western blot for panels E and F and a representative gel for panel F are shown in supplemental Fig. 1.