FIGURE 4.

Specificity of hnRNP L binding and activity. A, quantification of gel shift assays performed with purified recombinant hnRNP L and purified RNAs as indicated. B, representative gel shifts used for graph in panel A. For all panels, titration of protein is at 1, 3, 10, and 30 ng of hnRNP L per reaction. C, quantification of in vitro splicing assays in which purified recombinant hnRNP L was added to nuclear extract derived from resting cells and then incubated with minigene RNAs in which the middle exon contained wild-type exon 4, wild-type exon 5, or mutants of exon 4 as in Fig. 3. S.D. and p values are shown in supplemental Table 3.