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spIL-33 induces signal transduction in a dose-dependent manner. HMC-1 (A) and Raw 264.7 (B) cells were stimulated with various concentrations of recombinant spIL-33 for 15 min as indicated at the top. The phosphorylated or total signaling protein was detected with anti-phosphoprotein or total signaling protein as indicated at the right of each panel. The data represent one of three independent experiments.
