FIGURE 6.

HRG/TGZ induces DNA damage through oxidative stress. a, immunocytochemical staining of γ-H2AX was performed as described under “Experimental Procedures.” MCF-7 cells were incubated with the indicated compounds for 40 h. They were then stained for the presence of phosphorylated γ-H2AX (left panels). The nuclei were visualized by DAPI staining (right panels). A representative image is shown. C, control, untreated cells; H, HRG; T, TGZl; HT, HRG and TGZ. b, MCF-7 cells were incubated for 40 h with the indicated compounds. Cell lysates were analyzed by Western blot for the expression of phosphorylated γ-H2AX. C, control, untreated cells; H, HRG; T, TGZ; HT, HRG and TGZ.