FIGURE 3:

ER GIIα content in S. pombe cells expressing wild-type, mutant, or no GIIβ. Each lane was loaded with 250 μg of microsomal proteins of ΔGIIβ cells or ΔGIIβ cells expressing exogenous GIIβ or GIIβ-MRH* (MRH*). The membrane was blotted using mouse polyclonal anti-GIIα subunit (1:500) and rabbit polyclonal anti-CNX (1:100,000) primary antibodies. Goat HRP anti–mouse or –rabbit IgG (1:5000 and 1:30,000, respectively) were used as secondary antibodies. Reactions were detected by chemiluminescence.