Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jun 1;22(11):1824–1835. doi: 10.1091/mbc.E10-12-0929

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Kanda et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB”, “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 1: — Phosphorylation of TRPC6 Y284 is necessary for its trafficking to the plasma membrane. (A) Induction of membrane trafficking of TRPC6 by EGF. HEK293T cells expressing HA-TRPC6 were stimulated by EGF (200 ng/ml) for the indicated times. The cells were surface biotinylated with Sulfo-NHS-SS-Biotin, and the streptavidin-agarose–bound proteins were analyzed by Western blotting with α-TRPC6 antibody. The positions of the molecular-weight-marker proteins in kilodaltons are shown on the right side of the panel. A portion of each lysate (2%) was examined by Western blot to confirm the expression level of TRPC6 (bottom panel). (B) HA-TRPC6 expressed in HEK293T cells with or without constitutively active Fyn was immunoprecipitated and probed with anti-phosphotyrosine antibody. (C) HEK293T cells expressing HA-TRPC6 with or without Fyn were processed as in A. (D and E) Fyn and each of a series of phenylalanine substitution mutants of HA-TRPC6 were expressed in HEK293T cells (D) or in cultured podocytes (E), and the surface biotinylation assay for TRPC6 was performed as in A. Representative data from three to five independent experiments are shown.