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. 2011 Jun 1;22(11):1824–1835. doi: 10.1091/mbc.E10-12-0929

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© 2011 Kanda et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Tyrosine phosphorylation of TRPC6 in living cells and kidney glomeruli. (A) Lysates from HEK293T cells transfected with the indicated plasmids were probed with α-pY31 or α-pY284 antibody. (B) HEK293T cells expressing HA-TRPC6 were stimulated by EGF. α-HA immunoprecipitates were probed with α-phosphospecific TRPC6 antibodies. (C) Isolated rat glomeruli treated with or without 1 mM sodium vanadate for 30 min were blotted with α-pY284. For the indicated samples, glomeruli were pretreated with 10 mM PP2 for 15 min or 5 mM SU6656 for 60 min before treatment with vanadate.