Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jun 1;22(11):1864–1877. doi: 10.1091/mbc.E10-11-0904

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Raju et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB”, “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 5: — In synaptosome-enriched fractions, CBF-A specifically interacts with dendritically transported Arc, BDNF, and CaMKII mRNAs. (A) Preparation of synaptosome-enriched fractions. (B) P2 fractions were resolved by SDS–PAGE, stained with Coomassie, and analyzed on immunoblots with CBF-A (ICCI), PSD95, and histone H3 antibodies. (C) CBF-A can be specifically coprecipitated from synaptosomal preparations. P2 fractions were subjected to immunoprecipitation with the CBF-A (ICCI) antibody or nonspecific IgGs. Bound proteins were resolved by SDS–PAGE and CBF-A revealed on immunoblots with the ICCI antibody. Mock, protein A Sepharose incubated with P2 fraction in the absence of antibody. (D and E) In synaptosomal preparations, CBF-A is associated with BDNF, CaMKIIα, and Arc mRNAs. Total mRNA precipitated with anti–CBF-A (ICCI) antibody or nonspecific IgGs from synaptosomal preparations was isolated. The cDNA was analyzed with primers amplifying BDNF, CaMKIIα, BDNF, and α-tubulin mRNAs. Quantification of precipitated mRNA species was by densitometric analysis in at least three successful experiments.