FIGURE 4:

A specific set of DHHC PATs partially colocalized with stathmin 2 at the Golgi complex in hippocampal neurons. Hippocampal neurons transfected at 6 DIV with the indicated myc- or HA-tagged DHHCs were immunostained for endogenous stathmin 2 (St2, green), either HA or myc to visualize exogenous DHHCs (red), and CTR433 to visualize the Golgi complex (blue). (A) DHHC7 and -17 fully colocalized with CTR433 and displayed an extensive colocalization at the Golgi with endogenous stathmin 2 immunoreactivity, which was also present along neurites as a vesicle-like labeling. (B) As for DHHCs in (A), DHHC9, -12, and -15 colocalized with endogenous stathmin 2 at the Golgi (colocalization with CTR433 in Supplemental Figure S4), but were also present along neurites at vesicles not overlapping with those labeled for stathmin 2. DHHC22 colocalized with endogenous stathmin 2 at the Golgi but also showed an extra-Golgi labeling at the ER (colabeling with KDEL in Supplemental Figure S4). DHHC4 did not significantly colocalize with stathmin 2 but localized mainly to the ER (colabeling with KDEL in Supplemental Figure S4). Bars = 10 μM; boxed areas in the merged images are shown at higher magnification.