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. 2011 May 27;6(5):e20198. doi: 10.1371/journal.pone.0020198

Figure 3. Long-term Continuous CORT treatment increases VEGF protein levels in vitro and in vivo.

Figure 3

(A) CORT (CORT; 1 µM) was applied to mouse primary cortical neurons at DIV 5. VEGF protein levels were determined by western blotting analysis at 48 hand 72 h following CORT treatment. CON means DMSO treatment. Data represent mean±SE (n = 6) expressed as fold change in VEGF protein levels as compared to CON. *P<0.05 (Bonferroni's test). (B) VEGF protein levels in frontal cortex of mice treated with CORT (5 mg/kg) or vehicle control (CON; 0.45% hydroxypropyl-β-cyclodextrin) for 7 weeks were determined by western blot analysis. Data represent mean±SE (n = 5) expressed as fold change in VEGF protein levels as compared to CON. *P<0.01 (t test). (C) VEGF protein levels in serum samples collected from mice treated with CORT (CORT; 5 mg/kg) or vehicle control (CON; 0.45% hydroxypropyl-β-cyclodextrin) for 7 weeks were analysed by ELISA. Data represent mean±SE (n = 5–6) expressed as fold change in VEGF protein levels as compared to CON. *P<0.01 (t test).