Figure 4. Development of biological surface coating in a 96-h toxicity test at 2 mg/L nTiO2 in ISO-medium.
(A) 24 h, before 1st molting; (B) 25 h, about 1 h after 1st molting with arrow indicating renewed particle adhesions at filtration apparatus and the lower ventral gap; (C) 48 h; (D) 72 h; (E) 96 h; (F) ESEM-EDX picture of nTiO2 particle agglomerates colored in red on spine of D. magna following 48 h of exposure to, nTiO2 particles at 2 mg/L.