Figure 2. Circular permutation sites scanned in mCherry and mKate.

mCherry and mKate were numbered by primary amino acid sequences as shown in Fig. 1C . Open circles indicate sites with fluorescence, closed circles sites without fluorescence. Three highly homologous regions of circular permutation tolerant sites in mCherry and mKate: Loop 7–8 region located in the loop between the 7^th and 8^th β-strands and flanking sites on the β-strands, Loop 8–9 region located in the loop between the 8^th and 9^th β-strands and flanking sites on the β-strands, and Loop 9–10 region flanked by the 9^th and 10^th β-strands.