. 2011 May 27;6(5):e20505. doi: 10.1371/journal.pone.0020505

Table 4. Properties of circular-permutated mKate variants.

Protein^a	Absorption^b (A₅₈₈/A₄₄₂)	Quantum yield^c (588 nm)	Absorption^d (A₅₈₈/A₂₈₀)	Brightness of protein^e (% of mKate)
mKate	4.13	0.33	0.74	100
cp149-148	4.79	0.33	0.68	90.1
cp151-150	3.25	0.31	0.61	83.6
cp154-153	0.81	0.28	0.19	21.2
cp166-165	2.88	0.28	0.58	70.5
cp167-166	2.73	0.32	0.61	81.6
cp168-167	1.8	0.30	0.65	84.0
cp187-186	1.45	0.30	0.25	30.4
cp189-188	1.47	0.29	0.27	36.3

Red fluorescence of all circular-permutated mKate variants shown in this table, with a N-terminal leader peptide from pRSET vector, have the same excitation (588 nm) and emission (620 nm) maxima as mKate; whereas the published emission maximum of mKate are 635 nm [9], and 625 nm [24].

Absorption maxima for red and green chromophores are 588 nm and 442 nm, respectively.

Red fluorescence quantum yield at 588 nm excitation were measured using mKate as the reference standard.

Ratio of red chromophore absorption at 588 nm and protein absorption at 280 nm.

Fluorescence of cp-mKate relative to mKate with fixed protein concentration (BCA assay).