Table 5. Truncation of circular-permutated mKate variants.
Variant | Colony brightness 24 h at 37°C (% of mKate) | Colony brightness 72 h at 2–8°C (% of mKate) |
cp183-182* | 21.4 | 24.6 |
cp185-182 | 23.1 | 24.2 |
cp188-182 | 15.1 | 13.3 |
cp191-182 | 7.9 | 11.5 |
cp193-182 | 0 | 0 |
cp182-181* | 16.9 | 16.9 |
cp186-181 | 2.6 | 2.2 |
cp191-181 | 1.8 | 3.1 |
cp192-181 | 0.2 | 1 |
cp193-181 | 0 | 0 |
cp181-180* | 0 | 0 |
pRSET-A | 0 | 0 |
mKate | 100 | 100 |
*Original symmetric cp-mKate.