Table 2. List of analytes in the multiplex assay.
| Control group (n = 28) | ASD group (n = 28) | FDR- corrected P value | ||||
| Analytes | mean | SD | mean | SD | t value | |
| Group I | ||||||
| IL-1β | 1.1 | 0.8 | 1.7 | 0.8 | −2.616 | *0.049 |
| IL-1RA | 85.4 | 49.2 | 135.0 | 43.4 | −4.002 | *0.003 |
| IL-2 | BDR | BDR | - | - | ||
| IL-4 | 2.1 | 0.9 | 2.7 | 0.9 | −2.487 | 0.06 |
| IL-5 | 2.8 | 1.4 | 3.8 | 1.3 | −2.906 | *0.033 |
| IL-6 | 5.9 | 3.2 | 6.8 | 2.4 | −1.207 | 0.37 |
| IL-7 | 10.8 | 3.1 | 12.8 | 3.4 | −2.201 | 0.09 |
| IL-8 | 8.7 | 3.7 | 11.6 | 2.5 | −3.391 | *0.014 |
| IL-9 | 13.0 | 10.1 | 14.8 | 9.9 | −0.672 | 0.68 |
| IL-10 | 2.5 | 1.8 | 2.9 | 1.4 | −1.039 | 0.45 |
| IL-12 (p70) | 21.3 | 12.6 | 38.1 | 13.7 | −4.784 | *0.001 |
| IL-13 | 11.8 | 5.2 | 16.3 | 5.0 | −3.329 | *0.011 |
| IL-15 | BDR | BDR | - | - | ||
| IL-17 | 7.2 | 4.8 | 17.7 | 11.9 | −4.287 | *0.002 |
| Eotaxin | 86.7 | 50.8 | 107.6 | 35.3 | −1.789 | 0.16 |
| Basic FGF | BDR | BDR | - | - | ||
| G-CSF | 4.8 | 3.4 | 6.9 | 3.2 | −2.368 | 0.07 |
| GM-CSF | BDR | BDR | - | - | ||
| IFN-γ | 80.0 | 46.4 | 107.2 | 49.6 | −2.123 | 0.10 |
| IP-10 | 1912.2 | 3202.5 | 1075.1 | 322.0 | 1.376 | 0.33 |
| MCP-1 | 26.4 | 17.3 | 28.2 | 13.9 | −0.419 | 0.83 |
| MIP-1α | 6.5 | 2.6 | 6.7 | 2.8 | −0.227 | 0.91 |
| MIP-1β | 125.0 | 45.3 | 159.8 | 57.1 | −2.527 | 0.06 |
| PDGF-BB | 11053.2 | 3023.3 | 12465.3 | 1548.4 | −2.200 | 0.09 |
| RANTES | 6303.5 | 809.6 | 6103.5 | 598.4 | 1.051 | 0.46 |
| TNF-α | 8.6 | 9.1 | 18.0 | 19.6 | −2.316 | 0.08 |
| VEGF | 74.3 | 65.6 | 124.9 | 75.4 | −2.682 | 0.05 |
| Group II | ||||||
| CTACK | 555.8 | 138.7 | 606.1 | 145.8 | −1.324 | 0.33 |
| GRO-α | 60.5 | 38.3 | 99.0 | 47.4 | −3.347 | *0.013 |
| HGF | 213.1 | 83.5 | 266.0 | 66.9 | −2.619 | 0.06 |
| IFN-α2 | 38.4 | 11.1 | 38.1 | 8.2 | 0.150 | 0.92 |
| IL-1α | 0.5 | 0.4 | 0.6 | 0.4 | −0.990 | 0.47 |
| IL-2Rα | 59.6 | 20.0 | 56.3 | 22.2 | 0.579 | 0.76 |
| IL-3 | 17.1 | 16.6 | 17.3 | 9.9 | −0.051 | 0.96 |
| IL-12 (p40) | 43.4 | 23.2 | 56.2 | 26.8 | −1.907 | 0.15 |
| IL-16 | 210.8 | 90.0 | 220.6 | 73.1 | −0.447 | 0.83 |
| IL-18 | 60.3 | 24.3 | 61.3 | 17.2 | −0.171 | 0.93 |
| LIF | BDR | BDR | - | - | ||
| MCP-3 | 7.2 | 3.4 | 5.8 | 3.7 | 1.443 | 0.30 |
| M-CSF | 10.7 | 7.2 | 13.2 | 7.6 | −1.259 | 0.35 |
| MIF | 78.3 | 31.3 | 81.0 | 28.1 | −0.333 | 0.88 |
| MIG | 415.2 | 270.8 | 471.3 | 520.5 | −0.505 | 0.80 |
| β-NGF | 3.1 | 1.9 | 3.1 | 1.0 | 0.059 | 0.98 |
| SCF | 144.0 | 24.3 | 150.3 | 39.3 | −0.718 | 0.68 |
| SCGF-β | 29762.4 | 6331.6 | 32684.0 | 5613.8 | −1.827 | 0.16 |
| SDF-1α | 162.7 | 55.7 | 180.8 | 43.6 | −1.347 | 0.33 |
| TNF-β | 3.4 | 4.6 | 3.1 | 3.4 | 0.285 | 0.88 |
| TRAIL | 160.7 | 58.9 | 133.9 | 48.9 | 1.851 | 0.16 |
Concentrations of analytes are shown in [pg/ml]. Note the statistically significant difference between the two groups (*P<0.05 after FDR correction for multiple comparisons). Abbreviations: ASD, autism spectrum disorder; BDR, below the detection range; FDR, false discovery rate; and SD, standard deviation.