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(1) 50 µM LC4 in phosphate buffer at pH 7.4; (2) as in (1) but at pH 4.5; (3) 50 µM LC4 in SDS micelles at pH 7.4; (4) as in (3) but at pH 4.5. The experiments were carried out at room temperature in 80 mM phosphate buffer or 80 mM phosphate buffer containing 10 mM SDS.
