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. 2011 May 27;6(5):e20655. doi: 10.1371/journal.pone.0020655

Figure 4. Fusion competency of three Mfn2 mutants.

Figure 4

Mfn DKO cells were transfected with Myc-tagged wild type or the three Mfn2 mutants, and co-stained for Myc (red) and mitochondria (green). (A) Mitochondria in untransfected Mfn DKO cells are completely fragmented. UT: untransfected. (B) Mitochondrial morphology quantification. Mfn2-L374P and Mfn2-L408P restored tubular mitochondria similar to or more effectively than wild-type Mfn2. The majority of cells transfected with Mfn2-L441P still contained fragmented mitochondria, indicating that Mfn2-L441P is fusion incompetent. Error bars represent SEM. (C–C”) Mfn DKO cells expressing wild-type Mfn2. The transfected cell positive for Myc staining (C) contains tubular mitochondria (C'). (D–D”, E–E”) Mfn DKO cells transfected with Mfn2-L374P (D) and Mfn2-L408P (E) show tubular mitochondria (D', E'). (F–F”) Mitochondria in the Mfn DKO cell expressing Mfn2-L441P (F) remained fragmented (F'). T: transfected cells. Scale bar: 10 µm.