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. 2011 May 27;6(5):e20608. doi: 10.1371/journal.pone.0020608

Figure 1. Lysophosphatidate does not block the entry of Taxol treated MCF-7 cells into G2/M.

Figure 1

Panels A–E, cells were treated with BrdU for 1 h to label cells in S-phase. Cells were then incubated for 12 h with 10% delipidated serum in the presence or absence of 50 nM Taxol or 5 µM LPA (Protocol A). The progression of cells through S, G2/M and G1 phases was quantified (Panels B–E) from the FACS analysis (Panel A). DNA histograms were composed with CellQuest software. The histogram for time 0 for all treatments shows the same results, repeated for clarity of the figure. Results are means ± SD for three independent experiments.