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. 2011 May 27;6(5):e20608. doi: 10.1371/journal.pone.0020608

Figure 9. Involvement of LPA1/3 and LPA2 receptors in LPA-induced displacement of Taxol from polymerized tubulin in MCF-7 and MDA-MB-231 cells.

Figure 9

MCF-7 (Panel A) and MDA-MB-231 cells (Panel B) were preincubated for 26 h with [3H]Taxol as in Fig. 7. After this, 5 µM LPA or 1 µM VPC51299, a LPA1/3 receptor antagonist, was added as indicated and the incubations were continued for a further 12 h. Polymerized tubulin was then isolated and the distribution of [3H]Taxol was determined. Results are means ± SD for three independent experiments. *P<0.05 comparing cells incubated in the absence of LPA with those incubated with LPA and/or the LPA1/3 antagonist, VPC51299.