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Figure 3. Mg2+ alters ALDH2 activity in a complex manner.
The effect of Mg2+ ion concentration on enzyme activity. Reaction mixtures contained 0.25 µM ALDH2 (tetramer), 1 mM NAD+, 100 µM propanal, and the indicated Mg2+ concentrations in 40 mM HEPES buffer, pH 7.4.