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. Author manuscript; available in PMC: 2012 May 30.

Published in final edited form as: Chem Biol Interact. 2011 Jan 27;191(1-3):147–152. doi: 10.1016/j.cbi.2011.01.023

(A) The fluorescence waveforms collected during enzymatic catalysis are used to determine the changes in concentration of free and bound forms of NADH. In addition to the fluorescence from the free NADH (τ = 0.4 ns) and ALDH2-NADH complex (τ = 6.0 ns) observed in the K_D studies, a third fluorescent contributor (τ = 11.2 ns) is resolved in the presence of Mg²⁺. The reaction mixture contained 0.25 µM ALDH2, 1 mM NAD⁺, 100 µM propanal, and 6000 µM Mg²⁺. (B) The effect of Mg²⁺ ion concentration on relative fluorescence intensities reached at steady state conditions from the ALDH2-NADH complex and third fluorescent contributor.

(A) The fluorescence waveforms collected during enzymatic catalysis are used to determine the changes in concentration of free and bound forms of NADH. In addition to the fluorescence from the free NADH (τ = 0.4 ns) and ALDH2-NADH complex (τ = 6.0 ns) observed in the K_D studies, a third fluorescent contributor (τ = 11.2 ns) is resolved in the presence of Mg²⁺. The reaction mixture contained 0.25 µM ALDH2, 1 mM NAD⁺, 100 µM propanal, and 6000 µM Mg²⁺. (B) The effect of Mg²⁺ ion concentration on relative fluorescence intensities reached at steady state conditions from the ALDH2-NADH complex and third fluorescent contributor.