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. Author manuscript; available in PMC: 2012 May 30.
Published in final edited form as: Chem Biol Interact. 2011 Jan 27;191(1-3):147–152. doi: 10.1016/j.cbi.2011.01.023

Figure 5. Resolved spectral and temporal contributions from WTM of a sample containing 2.9 µM NADH, 0.25 µM ALDH2, and 6.0 mM Mg2+ in 40 mM HEPES buffer (pH 7.4).

Figure 5

Figure 5

(A) The resolved fluorescence spectra illustrates that the third contributor has an identical fluorescent spectrum to that of the ALDH2-NADH complex. (B) The resolved temporal contributions provide a measure of the relative amounts and the fluorescence lifetime of each contributor.